副溶血性弧菌重复序列-PCR分型研究

目的利用REP—PCR技术对副溶血性弧菌进行分子分型研究和亲缘关系的探讨。方法以基因外重复回文序列（REP）为引物，对20株副溶血性弧菌基因组DNA进行扩增，得到DNA指纹图谱，并利用SPSS11．5统计软件对DNA扩增图谱进行分析，做出聚类图，并与血清型进行比较分析。结果REP-PCR可以把20株菌分为7个型，优势菌型为A型和B型，分辨力指数为0．932。结论REP．PCR方法可以用于副溶血性弧菌分型分析，具有较好的分型能力，如果能将分子分型和血清分型两种方法联用，分辨率会更高。研究推断血清型01群与03群菌株之间亲缘关系密切。

Typig of Vibrio parahaemolyticus by Repetitive Element Sequence-based PCR

Objective REP-PCR method was used on typing research and genetic relationship research for Vibrio parahaemolyticus pathogens. Methods REP sequences used as the PCR primers. Twenty Vibrio parahaemolyticus strains were amplified and DNA fingerprints were gained. The dendmgrams were made by the computer software SPSS 11.5. And compared with serum typing. Results According to REP-PCR fingerprints, 20 Vibrio parahae- molyticus strains could be grouped into 7 patterns by computer analysis and A type was predominant. Discriminative index was 0.932. Conclusion REP-PCR is feasible for typing Vibrio parahaemolyticus strains. Vibrio parahaemolyticus strains, and indicate that the genetype of serovar 03 and O1 are closely related.