Improved alkaloid production in Catharanthus roseus suspension cell cultures by various chemicals |
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Authors: | Jian Zhao Wei-Hua Zhu Qiu Hu Xing-Wang He |
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Institution: | (1) Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, P.R. China;(2) Ren Ming Hospital, LinHe, Inner Mongolia, 015000, P.R. China |
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Abstract: | Fourteen chemicals were used to treat Catharanthus roseussuspension cell cultures to improve ajmalicine, catharanthine or serpentine biosynthesis. Ajmalicine production was increased by betaine (to 55 mg l–1), n-propyl gallate (to 27 mg l–1), succinic acid (to 31 mg l–1), malic acid (to 60 mg l–1) and tetramethyl ammonium bromide (to 64 mg l–1). Ajmalicine and catharanthine yields were about 5–6 fold higher than the control. A large portion (up to 50–85%) of total indole alkaloids was released into the medium. For maximal catharanthine production, the optimal doses of malic acid and tetramethyl ammonium bromide were 50 mg l–1and 120 mg l–1, respectively. The mechanisms which may be responsible for these treatment effects are discussed. |
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Keywords: | ajmalicine catharanthine Catharanthus roseus malic acid serpentine tetramethyl ammonium bromide |
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