Regeneration from long-term cell suspension cultures of tepary bean (Phaseolus acutifolius) |
| |
Authors: | A S Kumar O L Gamborg M W Nabors |
| |
Institution: | (1) Tissue Culture for Crops Project, Department of Biology, Botany Section, Colorado State University, 80523 Fort Collins, CO, USA |
| |
Abstract: | Plant regeneration has been achieved from long-term cell suspension cultures established from leaf derived callus of tepary bean (Phaseolus acutifolius). The proportion of densely cytoplasmic cells in suspension culture increased when cultured in the L-6 medium with 54 M NAA and 2 M KN. Filtration of the cells at each of five consecutive subcultures resulted in the isolation of a plant regenerating cell line (TB 686), which is being maintained in L-6 medium with 4.5 M 2,4-D and 2.3 M zeatin. Differentiated green cell aggregates were obtained when cells from maintenance medium were transferred to the same medium with 10 M BA. Embryo-like structures developed from these aggregates on L-6 medium with 2.3 M zeatin, 0.69 M GA3 and 1.5 M NAA. Plantlets regenerated from these structures when they were cultured on L-6 medium with 7.0 M NAA and 1.0 M KN. Plant regeneration from the cell line remained relatively constant for 270 days. Regenerated plants were grown to maturity in the greenhouse.Abbreviations BA
Benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- GA3
Gibberellic acid
- IPA
Isopentenyladenine
- KN
Kinetin
- NAA
Naphthaleneacetic acid
- AA
Amino acid medium (Toriyama and Hinita, 1985)
The research was sponsored by United States Agency for International Development, Washington D.C., Cooperative Agreement DAN-4137-A-00-4053-00 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|