Cadaverine Involved in Urate Degrading Activity (Uricase Activity) in Soybean Radicles |
| |
Authors: | Tajima Shigeyuki; Kato Natsuki; Yamamoto Yukio |
| |
Institution: | 1Department of Agricultural Chemistry, Kagawa University Miki-cho, Kita-gun, Kagawa 761-07, Japan
2Department of Agricultural Chemistry, Nagoya University Furo-cho, Chikusa-ku, Nagoya 464, Japan
3Department of Agronomy, Nagoya University Furo-cyo, Chikusa-ku, Nagoya 464, Japan |
| |
Abstract: | Cadaverine, a 5-carbon diamine, was identified as the cofactorof uricase activity previously found in soybean seedlings. Thesubstance purified from freeze dried hypocotyls was subjectedto liquid chromatography, mass spectrometry, 1H- and 13C-nuclearmagnetic resonance spectrometry for identification. The concentrationsof cadaverine in 3-day-old radicles and hypocotyls were 2.37mM and 5.09 mM, respectively. Other polyamine concentrationswere low. Biogenic polyamines (cadaverine, putrescine, spermidineand spermine) functioned as cofactors, whereas conjugated polyamines(tyramine and histamine) and amino acids had no effect. Theaddition of catalase to the assay system counteracted the effectof cadaverine. Peroxide at appropriate concentrations actedlike cadaverine with an identical Km value, suggesting thaturate degrading activity can be ascribed to the diamine oxidase-peroxidasesystem. (Received October 19, 1982; Accepted December 23, 1982) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|