Age-dependent metabolic changes in cultured human fibroblasts |
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Authors: | A L Muggleton-Harris R Defuria |
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Institution: | (1) MRC Experimental Embryology and Teratology Unit, Medical Research Council Laboratories, Woodmansterne Road, SM5 4EF Carshalton, Surrey, England;(2) Present address: Digital Analysis Corporation, 22091 Reston, Virginia |
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Abstract: | Summary The effects of metabolic poisons on the ATP content of cultured human skin fibroblasts at selected in vitro and in vivo ages
were studied. Potassium cyanide, iodacetemide, and Arsenate were used to inhibit ATP restoration by glycolysis and oxidative
phosphorylation. Cells treated with these metabolic poisons showed an age-dependent change in their ATP content. The decrease
in cellular ATP content after exposure to these drugs was taken as an estimate of ATP turnover. It was found that there was
a decrease in the ATP turnover with increasing population doubling level (i.e. in vitro age), and cells cultured from a 68-yr-old
donor had a lower ATP turnover than those cultured from a neonatal donor. This decreased ATP turnover correlates with a previous
finding of a decreased ability of “older” cells to be stimulated to migrate in culture and suggests that there is a metabolic
component to this age-related functional deficiency.
This work was supported by National Institutes of Health grants 2, RO1 EY02523 and 1 RO1 1, AGO 1212 awarded to A.L. Muggleton-Harris. |
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Keywords: | age related metabolic changes human fibroblasts |
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