Poly(ADP-ribose) polymerase gene on chromosome 1q: Early role in differentiation linked replication; gene on human chromosome 13q: Marker of carcinogenesis

We have focused in part on utilizing molecular aspects of the PADPRP gene sequences located on chromosome 1 q as well as on 13 to help clarify the biological role of these genes. With respect to chromosome 1 q, stably transfected cell lines have been established in-both Hela as well as 3T3-L1 preadipocyte cells, which express a reverse complementary transcription productin vivo to the exons coded for on the chromosome 1 locus. Also, in the case of the PADPRP-like gene on chromosome 13q, we have fine mapped this sequence and have observed in a linkage of deletion of this region with predisposition to various forms of cancer, especially in the African-American population.