<Superscript>13</Superscript>C-<Superscript>13</Superscript>C NOESY: A constructive use of <Superscript>13</Superscript>C-<Superscript>13</Superscript>C spin-diffusion期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

13C-13C NOESY: A constructive use of 13C-13C spin-diffusion

Authors:	Ivano?Bertini author-information" > author-information__contact u-icon-before" > mailto:bertini@cerm.unifi.it" title=" bertini@cerm.unifi.it" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Isabella?C.?Felli,Rainer?Kümmerle,Claudio?Luchinat,Roberta?Pierattelli

Affiliation:	(1) Magnetic Resonance Center and Department of Chemistry, University of Florence, Italy;(2) Bruker BioSpin AG, Fällanden, Switzerland;(3) Magnetic Resonance Center and Department of Agricultural Biotechnology, University of Florence, Italy

Abstract:	¹³C-¹³C NOESY experiments were performed under long mixing time conditions on reduced human superoxide dismutase (32 kDa, ¹⁵N, ^13C and 70% ²H labeled). ¹³C-¹³C couplings were successfully eliminated through post-processing of in-phase-anti-phase (IPAP) data. It appears that at mixing time _m of 3.0 s the spin diffusion mechanism allows the detection of 96% of the two-bond correlations involving C and C. The interpretation was confirmed by simulations. This approach broadens the range of applicability of ¹³C-¹³C NOESY spectroscopy.

Keywords:	¹³C direct detection Cu,Zn-SOD NOESY spin diffusion superoxide dismutase
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