| 肠道病毒ECHO25河南分离株基因特征分析 |
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| 引用本文： | 晁灵,黄学勇,李幸乐,许汴利.肠道病毒ECHO25河南分离株基因特征分析[J].病毒学报,2010,26(6). |
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| 作者姓名： | 晁灵 黄学勇 李幸乐 许汴利 |
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| 作者单位： | 郑州大学,公共卫生学院,郑州,450001;河南省疾病预防控制中心,郑州,450016 |
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| 基金项目： | 河南省医学科技攻关项目(项目号:200702016) |
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| 摘 要： | 首次对ECHO25病毒进行分子生物学分析,阐明ECHO25(Entric Cytopathic Human Orphanviruses Type25)病毒河南分离株的分子生物学特征及其与世界其它分离株的基因关系。逆转录-聚合酶链反应(RT-PCR)扩增出VP1蛋白编码基因并进行序列测定,将所测4株ECHO25病毒的VP1序列与GenBank上已发表的ECH-O25病毒VP1区进行同源性比较及遗传进化分析发现:河南省4株ECHO25与标准株JV-4核苷酸同源性为79.2%～80.1%,氨基酸同源性为89.0%～92.4%;河南省4株ECHO25核苷酸同源性为93.0%～99.0%,氨基酸同源性为92.4%～97.5%;HN-01分离株与HN-26分离株高度同源,其核苷酸同源性达99.0%;河南省4株ECHO25同属B1基因亚型。
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| 关 键 词： | ECHO 25 基因 序列分析 系统发生树 |
Genetic Characterization of Echovirus 25 Isolated from Henan |
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| CHAO Ling,HUANG Xue-yong,LI Xing-le,XU Bian-li.Genetic Characterization of Echovirus 25 Isolated from Henan[J].Chinese Journal of Virology,2010,26(6). |
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| Authors: | CHAO Ling HUANG Xue-yong LI Xing-le XU Bian-li |
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| Institution: | CHAO Ling1,HUANG Xue-yong2,LI Xing-le2,XU Bian-li2(1. College of Public Health Zhengzhou University,Zhengzhou 450001,China,2. Henan Center for Disease Control and Prevention,Zhengzhou,450016,China) |
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| Abstract: | This research firstly reported the molecular analysis of ECHO25(Entric Cytopathic Human Orphanviruses Type 25).To clarify molecular characteristics of the ECHO25 virus isolates in Henan Province and its relationship with the rest of world's isolates,the complete VP1 sequences of the 4 isolates in Henan were successfully amplified by RT-PCR and were compared with other ECHO25 isolates available from GenBank.Compared with the prototype strain JV-4,the nucleotide sequence identity was 79.2%-80.1%,and the amino... |
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| Keywords: | ECHO25 genome sequence analysis phylogenic tree |
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