Genetic diversity analysis of Cynodon dactylon (bermudagrass) accessions and cultivars from different countries based on ISSR and SSR markers |
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| Institution: | 1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Zhongshanmenwai, Nanjing 210014, China;2. Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Hainan University, Ministry of Education, Haikou 570228, China;3. College of Agronomy, Hainan University, Haikou 570228, China;4. College of Life Science, Qiongzhou University, Sanya 572000, China;1. CSIR - National Institute of Oceanography, Regional Centre, Kochi, 682 018, India;2. CSIR - National Institute of Oceanography, Regional Centre, Mumbai, 400 053, India;1. Laboratoire des RadiosIsotopes, Université of Antananarivo, BP 3383, 101, Antananarivo, Madagascar;2. Eszterházy Károly University, Eger, Hungary;3. Department of Biology, University of Iowa, Iowa City, IA, 52240 USA;4. IRD, UMR Eco&Sols, Laboratoire des RadiosIsotopes, Université of Antananarivo, BP 3383, 101, Antananarivo, Madagascar;1. Graduate School of Bioresources, Mie University, 1577 Kurima-machiya, Tsu, Mie Pref. 514-8507, Japan;2. Faculty of Bioresources, Mie University, 1577 Kurima-machiya, Tsu, Mie Pref. 514-8507, Japan |
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| Abstract: | ISSR and SSR markers were used to evaluate genetic diversity among 33 Cynodon dactylon accessions and 22 cultivars from four different countries in order to provide information on how to improve the utilization of bermudagrass germplasms. Eighty eight bands were amplified by nine SSR primer combinations and 236 bands were observed from 23 ISSR primers. The results showed that 97.7% of the SSR primers and 86.9% of the ISSR primers were polymorphic. The genetic similarity coefficients (GSC), gene diversity (He) and Shannon index (I) were 0.58–0.97, 0.27 and 0.41, respectively, for ISSR and 0.52–0.97, 0.29, and 0.43 for SSR. The UPGMA analysis clustered the 55 accessions (cultivars) into three groups. The cluster results produced by the ISSR data were close to the SSR data results. Analysis based on the combined ISSR and SSR data was more closely related to the geographical distribution of the tested germplasm. |
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