Analysis of the genetic diversity of garlic (Allium sativum L.) germplasm by SRAP |
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| Affiliation: | 1. Department of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat Arunachal Pradesh, India;2. ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, Maharashtra, India;3. Department of Fruit Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat Arunachal Pradesh, India;4. Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru-560 089, Karnataka, India |
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| Abstract: | ![]()
Cluster analysis and principal component analysis were used to investigate the genetic diversity of 40 garlic germplasms analyzed with 23 sequence-related amplified polymorphism (SRAP) primer combinations. A total of 130 polymorphic loci were detected among these germplasms, with an average of 5.65 polymorphic loci per SRAP primer combination. The percentage of polymorphic loci was 69.1%, whereas the mean effective number of alleles, the mean Nei's gene diversity, and the mean Shannon's information index were 1.4446, 0.2788, and 0.4365, respectively. Cluster analysis revealed that the 40 germplasms could be divided into 3 groups. The results of principal component analysis were consistent with those of unweighted pair-group method with arithmetic averages (UPGMA) clustering analysis. The Shannon-Weaver information index ranged from 0.2419 to 0.4202, indicating that the garlic germplasms had high genetic diversity. |
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| Keywords: | Garlic germplasms SRAP Genetic diversity Clustering analysis Principal component analysis |
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