响应面优化酶解—微波辅助提取桑叶多糖的工艺研究

采用响应面优化酶解——微波辅助法从桑叶中提取桑叶多糖的提取工艺。在单因素试验的基础上通过采用Box-Behnken方法，研究液固比、提取时间、提取温度对桑叶多糖提取率的影响。结果显示，拟合方程显著，最终确定桑叶多糖的最优提取条件为：酶含量2%、酶解pH6、酶解温度50℃、酶解时间20 min、液固比15 mL·g^-1、提取时间13 min、提取温度76℃，该条件下桑叶多糖的实际提取率为15.23%，与理论模拟值15.12%接近，建立的模型真实可靠。该方法用于提取桑叶中的多糖类成分，工艺简单、成本低，具有有较高的应用价值。

Response Surface Optimized Enzymatic Hydrolysis-microwave Assisted Extraction Process of Mulberry Leaf Polysaccharide

The response surface analysis was used to optimize the process of extracting polysaccharide from mulberry leaves by enzymatic hydrolysis-microwave assisted method.Based on the single factor test, the Box-Behnken method was used to study the effects of liquid-solid ratio, extraction time and extraction temperature on the extraction rate of mulberry leaf polysaccharides. The fitting equation was significant, and the optimal extraction conditions of mulberry leaf polysaccharides were finally determined as follows:enzyme content 2%, enzymolysis pH6, enzymolysis temperature 50℃, enzymolysis time 20 min, liquid-solid ratio 15 mL·g^-1, extraction time 13 min, and extraction temperature 76℃.Under these conditions, the actual extraction rate of mulberry leaf polysaccharide was 15.23%, which was closed to the theoretical simulation value of 15.12%. Therefore, the established model was true and reliable. The method can be used for extracting polysaccharide components in mulberry leaves with the advantages of simple process, low cost and high application value.