Harvest-inducibility of the promoter of alfalfa<Emphasis Type="Italic"> S</Emphasis>-adenosyl-<Emphasis Type="SmallCaps">l</Emphasis>-methionine:<Emphasis Type="Italic"> trans</Emphasis>-caffeoyl-CoA3-<Emphasis Type="Italic">O</Emphasis>-methyltransferase gene |
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Authors: | Email author" target="_blank">Jian?ZhangEmail author Larry?R?Erickson |
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Institution: | (1) Plant Agriculture Department, University of Guelph, Guelph, ON, N1G 2W1l, Canada;(2) Alberta Innovates-Technology Futures, Vegreville, AB, T9C 1T4, Canada |
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Abstract: | A major limitation on the expression of some foreign proteins in transgenic plants is the toxic effect of such proteins on
the host plant resulting in inhibition of normal growth and development. A solution to this problem is to control the expression
of genes for such proteins by means of inducible promoters, as is frequently done in microbial systems. A cDNA clone was obtained
from subtractive hybridization of non-harvested and harvested alfalfa leaf tissue, named hi12. The hi12 cDNA was identified as part of the S-adenosyl-l-methionine: trans-caffeoyl-CoA3-O-methyltransferase gene of alfalfa, a gene encoding an essential key enzyme in lignin synthesis. The hi12 gene was strongly induced by harvesting and wounding but not by heat shock. The promoter of the hi12 gene, isolated by genomic walking, contained several stress response cis-elements. Transgenic plants of tobacco and Medicago truncatula containing the GUS gene driven by the promoter showed GUS expression following harvesting, demonstrating the activity of
these regulatory regions in other plant species. |
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