水稻种子萌发过程中α 淀粉酶与萌发速率关系的分析

用丙烯酰胺凝胶电泳技术和3,5_二硝基水杨酸（DNS）测定还原糖法，对不同萌发时间农垦58F、农垦58S和农垦58种子胚中α_淀粉酶同工酶及其酶活性变化进行研究。0～36小时萌发过程中，农垦58F和农垦58S α_淀粉酶活性高于农垦58。胚中检测出五条酶。A2、A4和A5为上述种子共有酶带。A3为农垦58F和农垦58S特异性酶带。A1为农垦58特异性酶带。据此认为α_淀粉酶对水稻种子萌发速度起重要调控作用。A1和A3可能是调控种子发芽快慢的重要酶分子。

Analysis of the Relationship between α-Amylase and Germinating Rate of Rice Seeds during the Process of Seed Germination

The change of α_amylase activity and isoenzyme in embryos of Nongk en 58F, Nongken 58S and Nongken 58 were analyzed by assay of reducing sugars with 3,5_dinitrosalicylic acid(DNS) and acrylamide gel electrophoresis technique under the different germinating time. The α_amylase activity in the embryos of both Nongken 58F and Nongken 58S were higher than that of Nongken 58 from 0 to 36 h germinating time. Rice seed embryos had 5 isoenzyme bands(A1,A2,A3,A4,A5). A2,A4 and A5 were present in the embryos of Nongken 58F, Nongken 58S and Nongke n 58. A3 was specific for both Nongken 58F and Nongken 58S. A1 was specific for the embryos of Nongken 58. These results suggested a higher α_amylase activity be necessary for promoting fast germination of rice seeds, α-amylase isoenzyme A1 and A3 may play an important role in regulation of germination speed.