Induction of proteins during phage reactivation induced by UV irradiation, oxygen and peroxide in Bacteroides fragilis |
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| Authors: | JPetra Schumann David T Jones David R Woods |
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| Institution: | Council for Scientific and Industrial Research, Applied Microbial Genetics Research Unit, Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa |
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| Abstract: | Abstract Phage reactivation systems in Bacteroides fragilis were induced by far-UV irradiation, O2 and H2O2. These three treatments also induced the synthesis of 3, 6, and 4 protein bands, respectively, which were easily detectable by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Two proteins with apparent M r s of approx. 90 000 and 70 000 were induced by all three treatments. Caffeine completely inhibited UV- and O2-induced phage reactivation and prevented the synthesis of the M r 90 000 and M r 70 000 proteins. The results suggest that these two proteins may be involved in phage reactivation processes induced by UV, O2 and H2O2 in B. fragilis . |
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| Keywords: | Bacteroides fragilis phage reactivations effect of O2 H2O2 in anaerobes |
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