| 异育银鲫及其人工杂合种外源遗传物质的检测分析 |
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| 引用本文： | 丁军 谢岳峰. 异育银鲫及其人工杂合种外源遗传物质的检测分析[J]. 水生生物学报, 1993, 17(1): 22-26 |
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| 作者姓名： | 丁军 谢岳峰 |
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| 作者单位： | 中国科学院水生生物研究所，中国科学院水生生物研究所，中国科学院水生生物研究所，中国科学院水生生物研究所 武汉 430072，武汉 430072，武汉 430072，武汉 430072 |
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| 基金项目： | 国家自然科学基金(39170602)，湖北省自然科学基金(89J17)，国家淡水生态及生物技术开放实验室(FEBL)资助 |
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| 摘 要： | 采用 DNA 杂交的方法,对异育银鲫及其人工杂合种进行了外源 DNA 的检测分析,发现两个雌核发育系的异育银鲫及其人工杂合种的 DNA 与红鲤 DNA 片段间的分子杂交均有阳性斑点出现,这表明,在异育银鲫及其人工杂合种产生过程中,父本(红鲤)的 DNA 片段的确可以随机地掺入到母本细胞的 DNA 中,从而产生了异精效应,使其子代生长加快,并出现父本性状。
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| 关 键 词： | 人工杂合种 异育银鲫 遗传物质 |
THE ANALYSIS OF HETEROLOGOUS GENETIC MATERIALS IN ALLOGYNOGENETIC CRUCIAN CARP AND ITS ARTIFICIAL HYBRIDS |
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| Din Jun Xie Yuefeng Jiang Yigui Shan Shixin. THE ANALYSIS OF HETEROLOGOUS GENETIC MATERIALS IN ALLOGYNOGENETIC CRUCIAN CARP AND ITS ARTIFICIAL HYBRIDS[J]. Acta Hydrobiologica Sinica, 1993, 17(1): 22-26 |
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| Authors: | Din Jun Xie Yuefeng Jiang Yigui Shan Shixin |
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| Abstract: | In this paper,the heterologous genetic materials in allogynogenetic crucian carp and its artificial hybrids were analysed by using the method of dot hybridization of DNA.There were some positive dots when the DNA in two clones of allogynogenetic crucian carp and their ar- tificial hybrids were crossed with the pieces of DNA in red carp.Based on this result,it was suggested that,in the breeding of the allogynogenetic crucian carp and its artificial hybrids, some pieces of paternal DNA can integrate to maternal DNA at random,resulting in the effect of allogynogenesis',i.e.the allogynogenetic offspring have higher growth rate and some paternal characteristics. |
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| Keywords: | Allogynogenetic crucian carp Artificial hybrids of allogynogenetic crucian carp DNA hybridization Detection of paternal(red carp)DNA fragments |
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