Evaluation of Insulin and Ascorbic Acid Effects on Expression of Bcl-2 Family Proteins and Caspase-3 Activity in Hippocampus of STZ-Induced Diabetic Rats |
| |
| Authors: | Iraj Jafari Anarkooli Mojtaba Sankian Fatemeh Vahedi Shokoofeh Bonakdaran Abdol-Reza Varasteh Hossein Haghir |
| |
| Institution: | (1) Department of Anatomy and Neuroscience Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;(2) Department of Anatomy, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran;(3) Immunobiochemistry Lab, Immunology Research Center, Avicenna Research Institute, Mashad University of Medical Sciences, Ferdowsi Square, Bu-Ali Square, Mashhad, 9196773117, Iran;(4) Biotechnology Department, Razi Vaccine &; Serum Research Institute, Mashhad, Iran;(5) Department of Internal Medicine, Endocrine Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran |
| |
| Abstract: | Aims Effects of insulin and ascorbic acid on expression of Bcl-2 family proteins and caspase-3 activity in hippocampus of diabetic
rats were evaluated in this study. Methods Diabetes was induced in Wistar male rats by streptozotocin (STZ). Six weeks after verification of diabetes, the animals were
treated for 2 weeks with insulin or/and ascorbic acid in separate groups. Hippocampi of rats were removed and evaluation of
Bcl-2, Bcl-xL, and Bax proteins expression in frozen hippocampi tissues were done by SDS-PAGE electrophoresis and blotting. The Bcl-2,
Bcl-xL, and Bax proteins bands were visualized after incubation with specific antibodies using enhanced chemiluminescences method.
Caspase-3 activity was determined using the caspase-3/CPP32 Fluorometric Assay Kit. Results Diabetic rats showed increase in Bax protein expression and decrease in Bcl-2 and Bcl-xL proteins expression. The Bax/Bcl-2 and Bax/Bcl-xL ratios were found higher compared with non-diabetic control group. Treatments with insulin and/or ascorbic acid were resulted
in decrease in Bax protein expression and increase in Bcl-2 and Bcl-xL proteins expression. The Bcl-2/Bax and Bcl-xL/Bax ratios were found higher in treated groups than untreated diabetic group. Caspase-3 activity level was found higher in
diabetic group compared with non-diabetic group. Treatment with insulin and ascorbic acid did downregulated caspase-3 activity.
Conclusions Our data provide supportive evidence to demonstrate the antiapoptotic effects of insulin and ascorbic acid on hippocampus
of STZ-induced diabetic rats. |
| |
| Keywords: | Diabetes Insulin Ascorbic acid Hippocampus Apoptosis Rat-Caspase-3 Bcl-2 Bcl-xL Bax |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
