A new technique for producing recombinant baculovirus directly in silkworm larvae |
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Authors: | Kwang Sik Lee Bo Yeon Kim Yeon Ho Je Soo Dong Woo Hung Dae Sohn Byung Rae Jin |
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Institution: | (1) College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea;(2) School of Agricultural Biotechnology, Seoul National University, Seoul, 151-742, Korea;(3) Department of Plant Medicine, Chungbuk National University, Cheongju, 361-763, Korea |
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Abstract: | A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility
of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm
larvae. The recombinant virus was obtained from the hemolymph of injected larvae and the hemolymph then re-injected into the
larvae as an inoculum. This resulted in a high-level production of foreign protein in the silkworm larvae. This technique
produces easy and rapid recombinant protein production in silkworms. |
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Keywords: | Baculovirus expression vector Bombyx mori nucleopolyhedrovirus Recombinant baculovirus Cellulase Silkworm |
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