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| 旋扭山绿豆（Desmodiumintortum）凝集素的纯化与特性 | |
| 引用本文： | 彭建宗,程双奇,陈兆平,莫熙穆. 旋扭山绿豆（Desmodiumintortum）凝集素的纯化与特性[J]. Acta biochimica et biophysica Sinica, 1994, 0(6) |
| 作者姓名： | 彭建宗 程双奇 陈兆平 莫熙穆 |
| 作者单位： | 华南师范大学生物系 |
| 摘 要： | 旋扭山绿豆（Ｄｅｓｍｏｄｉｕｍｉｎｔｏｒｔｕｍ）凝集素的纯化与特性彭建宗，程双奇，陈兆平，莫熙穆（华南师范大学生物系，广州５１０６３１）关键词凝集素；旋扭山绿豆；亲和层析；糖蛋白豆科植物和根瘤菌之间的共生结瘤固氮关系早有报道。例如大豆只被Ｂ．仰。６。... |
| 关 键 词： | 凝集素；旋扭山绿豆；亲和层析；糖蛋白 |
Purification and Characterization of the Lectin from Desmodium intortum |
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| DENG Jian-Zhong,CHENG Shuang-Qi,OHEN Zhao-Ping and MO Xi-Mu. Purification and Characterization of the Lectin from Desmodium intortum[J]. Acta biochimica et biophysica Sinica, 1994, 0(6) | |
| Authors: | DENG Jian-Zhong CHENG Shuang-Qi OHEN Zhao-Ping MO Xi-Mu |
| Abstract: | The lectin in Desmodium intortum (DIL)was purified by affinity chromatography and gel filtration. The purified DIL exhibited a single band of molecular weight about 32 kd by SDS-PAGE. It is a glycoprotein and has a sugar content of 6.2%.DIL is an excellent mitogen. The transformation ratio of human peripheral blood lymphocytes is 87% and the cell division ratio is 15%.The results with 13 strains examined by the test of fluorescent label showed that FITO-DIL could combine specifically with 9 strains that nodulated Desmodium intortum but could not combine any of the other 4 strains that didn't nodulate D. intortum.It revealed a positive biological correlation between the lectin-binding capacily and the nodulating ability,illustrating that the DIL is important in the host-specific infection process which leads to nitrogen fixing symbiosis. |
| Keywords: | Lectin Desmodium intortum Affinity chromatography Glycoprotein |
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