| 阿拉伯胶酶产生菌株的筛选及其发酵培养基的优化 |
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| 引用本文： | 陈志锋,廖青青,应国清,梅建凤,陈建澍.阿拉伯胶酶产生菌株的筛选及其发酵培养基的优化[J].工业微生物,2011,41(5):69-72. |
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| 作者姓名： | 陈志锋 廖青青 应国清 梅建凤 陈建澍 |
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| 作者单位： | 1. 浙江工业大学药学院,浙江杭州310032;艾博生物医药有限公司,浙江杭州310018
2. 浙江工业大学药学院,浙江杭州,310032 |
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| 摘 要： | 从土壤中筛选产阿拉伯胶酶的微生物菌株,并通过紫外诱变选育后得到高产突变菌株ZHB05F,依据菌落和孢子形态特征初步鉴定为镰刀茵(Fusarium sp.).通过单因素试验,优化了产酶培养基的主要组分的浓度和pH值,得到最佳的产酶发酵培养基组成为:阿拉伯胶30 g/L,(NH4)2SO4 8 g/L,K2HPO4 1g/...
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| 关 键 词： | 阿拉伯糖 镰刀菌 培养基优化 |
Screening of high producing strain for arabic gum degrading enzyme and its optimization of fermentation medium |
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| CHEN Zhi-feng,LIAO Qing-qing,YING Guo-qing,MEI Jian-feng,CHEN Jian-shu.Screening of high producing strain for arabic gum degrading enzyme and its optimization of fermentation medium[J].Industrial Microbiology,2011,41(5):69-72. |
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| Authors: | CHEN Zhi-feng LIAO Qing-qing YING Guo-qing MEI Jian-feng CHEN Jian-shu |
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| Institution: | 1. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China; 2. Abon Biopharm Co., Ltd, Hangzhou 310018, China) |
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| Abstract: | A fungi strain, which could produce arabic gum degrading enzyme, was screened out from soil, and then was identified as Fusarium sp. by the characteristic of its morphology of clone and spore. After mutagenesis by ultraviolet, a high-yielding strain designated ZHB05F was selected out. Further, the composition of fermentation medium for enzyme production was optimized. The results showed that the optimal fermentation medium was consisted of arabic gum 30g/L,（NH）：SO4 8g/L,K2HPO4 1g/L,MgSO4·7H2O0.5g/L,KCl0.5g/L,FeSO4·7H2O0.01g/L,H3BO3 0.01g/L,pH5～6 with pH 5--6, the activity of enzyme was up to 147.7U/mL produced by the strain ZHB05F. |
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| Keywords: | arabinose Fusarium sp optimization of medium |
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