| 人精子甘露糖受体参与诱导卵母细胞皮质颗粒反应 |
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| 引用本文： | 江澍,林典梁,江一平. 人精子甘露糖受体参与诱导卵母细胞皮质颗粒反应[J]. 中国组织化学与细胞化学杂志, 2007, 16(5): 607-610 |
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| 作者姓名： | 江澍 林典梁 江一平 |
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| 作者单位： | 1. 福建医科大学细胞与发育工程研究中心,福州,350004;福建中医学院中西医结合系,福州,350108
2. 福建医科大学细胞与发育工程研究中心,福州,350004 |
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| 基金项目： | 国家自然科学基金;福建省自然科学基金;福建省教育厅科研项目 |
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| 摘 要： | 目的研究人精子甘露糖受体与卵母细胞皮质颗粒反应的关系。方法以亲和层析法纯化的人精子甘露糖受体(purified mannose receptor,pMR)作用去透明带金黄地鼠卵,继而用罗丹明偶联的兵豆凝集素(Tetramethylrhodam ine Isothiocyanate Labeled Lentil,TRITC-LCA)标记。通过荧光显微镜观察被标记的皮质颗粒及卵母细胞表面甘露糖基的变化情况。结果pMR作用卵母细胞后,卵母细胞内的皮质颗粒减少且细胞表面的甘露糖基数量增加。结论pMR作用于卵母细胞表面后,可触发皮质颗粒反应,并使皮质颗粒部分内容物转移到卵母细胞表面。
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| 关 键 词： | 甘露糖受体 人精子 皮质颗粒反应 卵母细胞 |
| 修稿时间： | 2007-03-14 |
THE CORTICAL GRANULE REACTION OF OOCYTES INDUCED BY MANNOSE RECEPTOR OF HUMAN SPERMATOZOA |
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| Jiang Shu,Lin Dianliang,Jiang Yiping. THE CORTICAL GRANULE REACTION OF OOCYTES INDUCED BY MANNOSE RECEPTOR OF HUMAN SPERMATOZOA[J]. Chinese Journal of Histochemistry and Cytochemistry, 2007, 16(5): 607-610 |
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| Authors: | Jiang Shu Lin Dianliang Jiang Yiping |
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| Abstract: | Objective To investigate the relationship between the purified mannose receptor(pMR) and the cortical granule reaction of oocytes.Methods The zona free hamster eggs were pre-incubated with pMR isolated from motile human sperms by mannose-agarose gel affinity chromatography.Tetramethylrhodamine Isothiocyanate Labeled Lentil(TRITC-LCA) was used to label the cortical granules(CGs) and surface mannose,and observed by fluorescence microscopy.Results After pre-incubation with pMR,the CGs of the oocyte decreased,but the mannose increased on the oolemma.Conclusion The results indicate that pMR can induce the cortical granule reaction,and the matrix of CGs might migrate to the surface of the oolemma after pMR pre-treatment. |
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| Keywords: | Mannose receptor Human sperm Cortical granule reaction Oocyte |
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