Influence of cysteine proteinase inhibitors on platelet and plasma components of blood coagulation system |
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Authors: | Ya N Kotova E A Kostanova M A Rozenfel’d E I Sinauridze M A Panteleev F I Ataullakhanov |
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Institution: | (1) Duke University School of Medicine, Durham, NC, USA |
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Abstract: | Factor XIIIa plays an important role in stabilization of formed fibrin clot during blood coagulation. Recent studies proved
that factor XIIIa affects formation of coated platelets, which are highly procoagulant and characterized by a high level of
alpha-granular proteins on their surface and expose surface phosphatidylserine after platelet activation. The ability of newly
found cysteine proteinase inhibitors (CPIs) from plants to affect thiol group of the factor XIIIa active centre was recently
discovered. Here, the effect of CPIs on the formation of coated platelets and activity of plasma components during blood coagulation
process was investigated. It was found that CPIs dose-dependently decreased the fraction of coated platelets in the total
platelet population during platelet activation and decreased endogenous thrombin potential (ETP) by 40% for thrombin generation
in platelet-rich as well as in platelet-poor plasma. Such decrease of ETP could not be explained by the CPIs influence on
factor XIIIa. Investigation of the effects of these inhibitors on factor Xa and thrombin activity has shown that CPIs dose-dependently
inhibited their activity and might cause an ETP decrease. Thus, the obtained data indicated that CPIs affected both platelet
and plasma components of blood coagulation system. |
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