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Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter |
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| Authors: | J J M Janssen W J M van de Ven W A H M van Groningen-Luyben J Roosien J M Vlak W J de Grip |
| Institution: | (1) Department of Biochemistry, Center of Eye Research, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands;(2) Dept. of Biochemistry, Molecular Oncology Section, University of Nijmegen, St. Adelbertuspl. 1, 6500 HB Nijmegen, The Netherlands;(3) Dept. of Virology, Agricultural University, 6709 PD Wageningen, The Netherlands |
| Abstract: | In vitro expression of cDNA encoding bovine opsin is accomplished using the baculovirus expression vector system. Full-length opsin was synthesized which was recognized by poly- and monoclonal antisera raised against bovine rhodopsin. Upon infection with a recombinant virus, 1×106 insect cells produced up to 3  g opsin. Incubation of the in vitro synthesized opsin with 11-cis retinal produced a hydroxylamine-stable, photosensitive pigment.Abbreviations dpi
days post infection
- pfu
plaque forming units
- AcNPV
Autographa californica nuclear polyhedrosis virus |
| Keywords: | baculovirus expression system immunocytochemistry in vitro expression opsin regeneration |
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