条斑紫菜藻红、藻蓝蛋白逐级放大的纯化工艺

采用“破碎-盐析-层析”的方法纯化条斑紫菜藻胆蛋白，并在提取规模上逐步放大。首先在综合比较凝胶层析去盐效率后，从Sephadex G-25、G-100、S-300和CL-6B中选择G-25作为实验流程中的去盐填料，其次将提取流程的初试原料条斑紫菜量逐步放大，选取了1g、20g和400g三个量，结果表明随着初试紫菜量逐步放大，最终所得藻胆蛋白中吸收光谱纯度>3.2的蛋白产率依次提高，其中400g冻干紫菜的藻红蛋白产率为0.323％，藻蓝蛋白产率为0.148％。由此认为该实验工艺流程具有规模放大的潜力，这为高纯度藻胆蛋白的规模生产提供了一条可行的方案。

The Establishment of Scale-up Isolation Procedure of Phycoerythrin and Phycocyanin from Porphyra yezoensis

The way of " extracting-sahing-chromatography" was used to purify the phycoerythrin and phyeoeyanin from Porphyra yezoensis in process scale-up. First, by comprehensive comparison of efficiency, the Sephadex G-25 was selected from four resins (Sephadex G-25 、G-100、S-300 and CL-6B) as the best choice used in crude extract desalting of phycobiliprotein. Then the preparation process of phycobiliprotein was scaled-up with raw material(Porphyra yezoensis) increased from 1 g to 20g, and finally to 400g. The results indicated that the yields of purified phyeoerythrin and phycocyanin (absorption spectra purity above 3. 2) increased during according to process scale-up,with 0.323% phycoerythrin and 0. 148% phycocyanin obtained from 400g frozen Porphyra yezoensis blades respectively. It is no doubt that the process involved in the experiment is a potential way for large scale preparation of phyeobiliproteins of high purity.