Improvement in stability of an immobilized fungal laccase |
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| Authors: | Andrzej Leonowicz Jawed M Sarkar Jean-Marc Bollag |
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| Institution: | (1) Laboratory of Soil Biochemistry, Department of Agronomy, The Pennsylvania State University, 16802 University Park, PA, USA;(2) Present address: Department of Biochemistry, M. Curie-Sklodowska University, Lublin, Poland;(3) The Pennsylvania State University, 119 Tyson Building, 16802 University Park, PA, USA |
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| Abstract: | Summary A laccase of the basidiomyceteTrametes versicolor was immobilized on porous glass beads that were activated with 3-aminopropyltriethoxysilane and glutaraldehyde. The support immobilized 100% of the enzyme, whereupon 90% of the original activity was retained. After immobilization, the enzyme was active in a wider pH and temperature range, and its heat stability and reuse were greatly improved compared to those of the free laccase. The immobilized enzyme was found reusable in treating different substrates, either recycled alone or in a sequential order. |
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