Genetic variation in somatic embryogenic response in open-pollinated families of black spruce |
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Authors: | W M Cheliak K Klimaszewska |
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Institution: | (1) Forestry Canada, Petawawa National Forestry Institute, P.O. Box 2000, KOJ 1JO Chalk River, Ontario, Canada;(2) Present address: Science Directorate 21st Floor, 351 St. Joseph Blvd., K1A 1G5 Hull, Quebec, Canada |
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Abstract: | Summary Zygotic embryos from open-pollinated seeds of 20 black spruce (Picea mariana) families were used to investigate the proportion of genotypes that would give rise to embryogenic tissue (ET) and mature somatic embryos. Eighty-five percent of the maternal genotypes gave rise to embryogenic tissue. Within-family rates of ET induction ranged from 0 to 17%, with an average of 8%. The largest proportion of variation was among families, indicating the additive nature of the genetic variation. On a medium with 6% sucrose and 3.7 M ABA, 90% of the embryogenic lines gave rise to abundant (>100/100 mg of ET), well-formed, mature somatic embryos. A medium with 2% sucrose, without 2,4-D, was used to germinate the mature somatic embryos. These were grown in the greenhouse and have now been established in field trials. |
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Keywords: | Somatic embryogenesis Picea mariana Biotechnology |
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