小檗碱对肠易激综合征大鼠肠道菌群的影响

目的通过口服小檗碱(Berberine,BBR)治疗大鼠肠易激综合征(IBS),初步研究小檗碱对IBS大鼠肠道菌群的影响。方法用避水应激试验制作大鼠IBS模型,分别给予模型大鼠小剂量(25mg/kg)小檗碱、大剂量(100mg/kg)小檗碱和利福昔明干预治疗10d,检测大鼠肠道菌群丰度、生物多样性及菌群结构组成的变化。结果避水应激试验造模后模型组大鼠排便次数增多、腹外斜肌EMG明显升高,肠道可见微炎症表现。小檗碱干预后,IBS大鼠排便增多情况、内脏高敏感性和肠道微炎症情况均显著改善,其中大剂量小檗碱组优于小剂量组。经小檗碱干预后,大鼠肠道菌群多样性显著降低,大剂量小檗碱抑菌作用强于小剂量小檗碱。大剂量小檗碱可显著提高IBS大鼠肠道乳杆菌科细菌的比例,同时降低肠杆菌科细菌的比例。结论小檗碱可以显著降低IBS大鼠内脏高敏感性,改善肠道微炎症,减少IBS大鼠排便增多症状。小檗碱可以调节肠道菌群丰度和多样性,且剂量越大,抑菌作用越明显。肠道乳杆菌科细菌在小檗碱治疗肠易激综合征的过程中可能起一定作用。

The effect of berberine on intestinal microflora in IBS rats

Abstract: Objective To explore the effect of berberine on the intestinal flora of IBS rats. Methods The rat models of IBS were made through water avoidance stress test and treated with 25 mg/kg berberine, 100 mg/kg berberine and Rifaximin respectively for 10 days. The changes in the population abundance, biological diversity and the colony structure of the intestinal flora were detected. Results After berberine intervention, the times of defecation and EMG of external abdominal oblique muscle in the IBS model group significantly reduced, and the intestinal micro-inflammation disappeared, with the high dose group being superior to the low dose group. After berberine intervention, the diversity of intestinal microflora of rats decreased, with the high dose groups being superior to low dose group. High dose of berberine significantly improved the proportion of Lactobacteriaceae but reduced that of Enterobacteriaceae. Conclusion Berberine can significantly prevent gut inflammation, visceral hyperalgesia and attenuates diarrhea symptoms in IBS rats. Berberine can regulate the abundance and diversity of intestinal flora in a dose dependent manner. Intestinal Lactobacilli may play a role duringthe treatment of irritable bowel syndrome with berberine.