Electroporation of pKK1 silver-resistance plasmid fromPseudomonas stutzeri AG259 intoPseudomonas putida CYM318 |
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| Authors: | Dr J T Trevors M E Starodub |
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| Institution: | (1) Department of Environmental Biology, Rm. 316, Chemistry-Microbiology, University of Guelph, N1G 2W1 Guelph, Ontario, Canada |
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| Abstract: | Plasmid pKK1 (49.4 Mda), which encodes for Ag+ resistance, was isolated fromPseudomonas stutzeri AG259 (pKK1) and introduced intoPseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1,P. putida CYM318 became resistant to AgNO3. This demonstrated that electroporation is a useful method to introduce a nonconjugative metal-resistance plasmid into a bacterial strain and to conduct further comparative research on Ag accumulation. |
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