Anthocyanin production in callus cultures of roselle (Hibiscus sabdariffa L.) |
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Authors: | Hajime Mizukami Kaomi Tomita Hiromu Ohashi Noboru Hiraoka |
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Institution: | (1) Faculty of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852 Nagasaki, Japan;(2) Niigata College of Pharmacy, 5-13-2 Kamishinei-cho, 950-21 Niigata, Japan |
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Abstract: | Callus tissues derived from seedlings of roselle (Hibiscus sabdariffa L.) were shown to produce two cyanidin glycosides as major anthocyanin pigments. Both callus growth and anthocyanin synthesis were remarkably stimulated by 2,4-dichlorophenoxyacetic acid. The highest anthocyanin yield was observed when 1 M 2,4-D in combination with 0.1–1 M kinetin was supplemented to the culture medium. In contrast, gibberellic acid showed inhibitory effect on anthocyanin production.Abbreviations LS
Linsmaier and Skoog
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
1-naphthaleneacetic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- GA
gibberellic acid |
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Keywords: | |
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