Molecular cloning and sequencing analysis of a β-tubulin gene from Lupinus albus |
| |
Authors: | Tatiana D Vassilevskaia Cândido Pinto Ricardo Claudina Rodrigues-Pousada |
| |
Institution: | (1) Laboratório de Genética Molecular, Instituto Gulbenkian de Ciência, Apartado 14, 2781 Oeiras, Portugal;(2) Laboratório de Bioquímica, Centro de Tecnologia Química e Biológica, Apartado 127, 2780 Oeiras, Portugal |
| |
Abstract: | Genomic -Dash library constructed from Lupinus albus nuclear DNA was screened using a fragment of the -tubulin cDNA ( 8–31) clone of Chlamydomonas reinhardtii as probe. One of the positive recombinant phages was isolated, subcloned and analysed by sequencing. We present here nucleotide and derived amino acid sequences of the -tubulin gene, designated as L1 and identified by similarity with other -tubulins. The L1-encoded protein reveals a very high degree of similarity with other plant tubulins and contains consensus sequences for binding guanine base, phosphate and Mg2+. Northern analysis of total RNA isolated from roots, leaves, flowers and pools revealed that Lupinus albus -tubulin genes are constitutively expressed in all studied plant tissues. |
| |
Keywords: | -tubulin" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-tubulin Lupinus albus |
本文献已被 SpringerLink 等数据库收录! |
|