HPLC法测定银州柴胡中五种皂苷的含量

首次建立HPLC法同时测定银州柴胡中柴胡皂苷a、c、d、e和f五种皂苷含量的方法。流动相为乙腈-水梯度洗脱,柱子为HibarRT RP-18(4.6 mm×250 mm,5μm),流速1.0 mL/min,柱温30℃,检测波长210 nm。柴胡皂苷a、c、d、e和f的线性范围及相关系数分别为:21.78～43.56μg(r=0.9993),3.94～9.85μg(r=0.9994),20.68～51.70μg(r=0.9998),1.67～5.57μg(r=0.9997),1.91～6.70μg(r=0.9992);柴胡皂苷a、d的加样回收率分别为101.5%和98.5%。该方法操作简便,结果准确,重现性好,为柴胡药材多指标质量分析方法的建立和银州柴胡药用提供了参考依据。

Determination of five saikosaponins in Bupleurum yinchowense by HPLC

A method was established for determining five saikosaponins simultaneously,including saikosaponins a,c,d,e and f in the root of Bupleurum yinchowense by HPLC for the first time.Separation was achieved by A Hibar RT C18 column(250 mm×4.6 mm,5 μm) using acetonitrile and water as the mobile phase with gradient elution at the f1ow rate of 1.0 mL/min,column temperature at 30 ℃ and the wavelength of UV detection was 210 nm.There were good linear correlations between the studied concentrations of the five saikosaponins and their chromatographic peak areas,and the correlation coefficients were 0.9992-0.9998.The recoveries of saikosaponins a and d were 101.5 and 98.5%,respectively.The RSD of precision and accuracy was less than 3%.This method is simple,accurate and reproducible,and also provides a reference for establishing the multiple-value quality control method for Radix Buplerui and the use of B.yinchowense as a medicine.