| 芦丁与人血清白蛋白相互作用的紫外可见光谱特性研究 |
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| 引用本文： | 黄汉昌,姜招峰. 芦丁与人血清白蛋白相互作用的紫外可见光谱特性研究[J]. 天然产物研究与开发, 2011, 23(3): 476-481 |
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| 作者姓名： | 黄汉昌 姜招峰 |
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| 作者单位： | 1. 北京联合大学应用文理学院
2. 北京联合大学生物活性物质与功能食品北京市重点实验室,北京,100191 |
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| 基金项目： | 北京联合大学应用文理学院科学研究发展基金;北京市教育委员会科技计划项目资助 |
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| 摘 要： | 本文通过测定芦丁与HSA相互作用前后的紫外可见吸收光谱、圆二色性及人血清白蛋白(HSA)的荧光特性,研究了芦丁与HSA结合作用。结果表明,芦丁在紫外区有三个特征的吸收峰(264.0、285.5及354.5nm)、在330～300 nm及300～230 nm处显示圆二色性,HSA引起芦丁紫外可见吸收光谱波峰红移;芦丁与HSA相互作用后,不引起HSA二级结构的改变,但对其三级结构有影响,同时对HSA荧光激发及发生光谱最大峰位及幅度有影响。
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| 关 键 词： | 芦丁 人血清白蛋白 紫外吸收 圆二色性 荧光光谱 |
The UV-Vis Spectrum Analysis on the Interaction between Rutin and Human Serum Albumin |
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| HUANG Han-chang,JIANG Zhao-feng. The UV-Vis Spectrum Analysis on the Interaction between Rutin and Human Serum Albumin[J]. Natural Product Research and Development, 2011, 23(3): 476-481 |
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| Authors: | HUANG Han-chang JIANG Zhao-feng |
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| Affiliation: | HUANG Han-chang1,JIANG Zhao-feng2 1College of Arts and Science of Beijing Union University,2Beijing Key Laboratory of Bioactive Substances and Functional Foods of Beijing Union University,Beijing 100191,China |
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| Abstract: | In order to inveatigate the interaction between rutin and human serum albumin(HSA),this article determined the ultraviolt-visible(UV-Vis) absorption spectrum,circular diachroism and HSA fluorescence spectrum,and further to analyse the difference between the spectra before and after rutin and HSA mixed.The results indicated that rutin shows three particular UV absorption-peak at the wavelength 264.0,285.5 and 354.5 nm and circular diachroism at the wavelength ranges 330~300 nm and 300~230 nm.When rutin inter... |
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| Keywords: | rutin Human Serum Albumin(HSA) UV-Vis absorption Circular Diachroism fluorescence |
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