Diffusing Wave and Ultrasonic Spectroscopy of Rennet-Induced Gelation of Milk in the Presence of High-Methoxyl Pectin |
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Authors: | Alexandra Acero Lopez Milena Corredig Marcela Alexander |
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Institution: | (1) Department of Food Science, University of Guelph, N1G2W1 Guelph, ON, Canada; |
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Abstract: | Rennet-induced aggregation was studied in milk systems containing high-methoxyl pectin (HMP) using ultrasonic and diffusing
wave spectroscopy. These two techniques allow for in situ measurements of sol–gel transitions without the need for dilution.
At low HMP concentrations, the casein micelles aggregation behavior was similar to that of skim milk, although changes could
be noted in the microstructure of the renneted gels. At HMP concentrations between 0.1 and 0.15%, phase-separation kinetics
were slower than the rennet-induced aggregation, and different microstructures formed caused by different dynamics of interactions
between casein micelles present in HMP-depleted flocs. Higher amounts of HMP failed to create a continuous gel, as phase separation
occurred at a faster rate than rennet aggregation. These results highlight the importance of non-invasive techniques in the
study of concentration-dependent phase separating and aggregating systems, as only with observations in situ is it possible
to determine new ways to control the structuring of protein–polysaccharide mixed systems. |
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Keywords: | |
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