Heterologous expression of murine lymphotoxins in Escherichia coli and preparation of antibodies] |
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| Authors: | V G Korobko V E Bo?chenko D V Kuprash R L Turetskaia S A Nedospasov |
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| Institution: | Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. |
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| Abstract: | Genes encoding fragments of polypeptide chains of murine lymphotoxins (LT), namely, LT-alpha truncated from the N-terminus and the LT-beta extracellular domain, containing N-terminal hepta- and hexahistidine epitopes, respectively, were expressed in E. coli cells. The recombinant proteins purified by metallochelate chromatography were used to obtain polyclonal antibodies that specifically recognize murine LT. |
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