A simple method for preparing physiologically active mitochondria from plant leaves rich in oils and phenolics |
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| Authors: | Keisuke Kohmoto Kazuya Akimitsu Tetsuyuki Kohguchi Hiroshi Otani John M Gardner |
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| Institution: | (1) Faculty of Agriculture, Tottori University, 680 Tottori, Japan;(2) The Citrus Research and Education Center, 33850 Lake Alfred, FL, USA;(3) Present address: Biological Disease Control, Allied Chemical Corp., Crop Science Laboratory, P.O. Box 6, 13209 Solvay, NY, USA |
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| Abstract: | Amberlite XAD-7, a nonionic polyacrylate adsorbent, was found to be a very effective protectant for isolating mitochondria from tissues rich in oils and phenolics. Physiologically active, well-coupled mitochondria were successfully prepared from young green leaf tissues of citrus, apple, pear and tobacco.Abbreviations DNP
2,4-dinitrophenol
- CCCP
carbonyl cyanide m-chlorophenylhydrazone
- BSA
bovine serum albumin
- PVP
polyvinylpyrrolidone |
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