Resistivity of Red Blood Cells Against High-Intensity, Short-Duration Electric Field Pulses Induced by Chelating Agents |
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Authors: | H Mussauer VL Sukhorukov A Haase U Zimmermann |
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Institution: | Lehrstuhl für Biotechnologie, Biozentrum, Universit?t Würzburg, Am Hubland, D-97074 Würzburg, Germany, DE Lehrstuhl für Experimentelle Physik (Biophysik), Universit?t Würzburg, Am Hubland, D-97074 Würzburg, Germany, DE
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Abstract: | The interaction of human red blood cells (RBCs) with diethylenetriamine-pentaacetic acid (DTPA) or its Gd-complex (Magnevist,
a widely used clinical magnetic resonance contrast agent containing free DTPA ligands) led to the following, obviously interrelated
phenomena. (i) Both compounds protected erythrocytes against electrohemolysis in isotonic solutions caused by a high-intensity
DC electric field pulse. (ii) The inhibition of electrohemolysis was observed only when cells were electropulsed in low-conductivity
solutions. (iii) The uptake of Gd-DTPA by electropulsed RBCs was relatively low. (iv) (Gd-) DTPA reduced markedly deformability
of erythrocytes, as revealed by the electrodeformation experiments using high-frequency electric fields. Taken together, the
results indicate that (Gd-) DTPA produce stiffer erythrocytes that are more resistant to electric field exposure. The observed
effects of the chelating agents on the mechanical properties and the electropermeabilization of RBCs must have an origin in
molecular changes of the bilayer or membrane-coupled cytoskeleton, which, in turn, appear to result from an alteration of
the ionic equilibrium (e.g., Ca2+ sequestration) in the vicinity of the cell membrane.
Received: 19 January 1999/Revised: 1 April 1999 |
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Keywords: | : Erythrocytes — Deformability — DTPA — Electropermeabilization — Electrodeformation — Electrorotation |
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