A new sub-sampling method for analysis of air samples collected with the Andersen single-stage sampler |
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| Authors: | Catharine M. Catranis Susan E. Anagnost Lianjun Zhang Shuang Zhou Analie Fernando Shannon Morey C. J. K. Wang |
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| Affiliation: | (1) American Type Culture Collection, 10801 University Blvd, Manassas, VA 20110, USA;(2) Faculty of Construction Management and Wood Products Engineering, State University of New York College of Environmental Science and Forestry, Syracuse, NY 13210, USA;(3) Faculty of Forest and Natural Resources Management, State University of New York College of Environmental Science and Forestry, Syracuse, NY 13210, USA;(4) Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA;(5) Faculty of Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, Syracuse, NY 13210, USA |
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| Abstract: | A protocol for bioaerosol collection was developed that provides not only accurate predictions of fungal concentration, but also improves species recovery. Random transfer of a subset of 50 of the 400 impaction points from Andersen single-stage bioaerosol sampling plates results in subcultures that are accurate predictors of fungal concentration (CFU/m3), when compared to duplicate untouched Andersen plates. A linear regression model was developed to estimate CFU/m3 from the colonies counted on the Random-50 plates. The random transfer to five plates (“Random-50” plates), allows large numbers of fungi to be recovered and identified, including slow-growing fungi that otherwise would be masked by fast-growing fungi. |
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| Keywords: | Bioaerosols Colony forming units Fungi Indoor environmental quality Model |
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