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Stability of plasmid pBR 322 in escherichia coli nf 161 (rela+) and nf 162 (rel a) grown in a methionine limited chemostat |
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| Authors: | K H Hofmann T Schweder U Reinhardt M Hecker |
| Abstract: | The stability of the plasmid pBR 322 was measured in E. coli NF 161 (rel A+) and NF 162 (rel A) grown in a methionine limited chemostat. A significant plasmid loss occurred only in E. coli NF 162 at a low dilution rate and if the fermenter had been inoculated with cells from the stationary phase. Obviously, this behaviour resulted from the high expression of β-lactamase due to amplification of the pBR 322 DNA in the inoculum cells of E. coli NF162. |
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