一株异养硝化-好氧反硝化皱褶念珠菌(Diutina rugosa)的分离及脱氮特性

为了获得异养硝化-好氧反硝化菌株,从养殖池塘污泥中分离筛选到一株具有异养硝化-好氧反硝化能力的酵母菌,命名为DW-1。经形态学观察和26S rDNA序列分析后鉴定为皱褶念珠菌DW-1(Diutina rugosa DW-1)。以氨氮为唯一氮源,初步探讨了碳源、C/N、初始pH值、培养温度、摇床转速对菌株DW-1除氮性能的影响。结果表明,在以乙酸钠为唯一碳源,C/N为25,pH为6.0、适宜培养温度为32℃、转速为170 r/min的条件下,菌株DW-1氨氮降解率和总氮去除率分别为94.94%、48.69%,而整个过程中亚硝氮积累量仅为0.067 mg/L。皱褶念珠菌DW-1的异养硝化-好氧反硝化特性表明其在降解含氮废水方面具有良好的应用前景。

Isolation and Characterization of Heterotrophic Nitrification-Aerboic Denitrification Diutina rugosa

In order to obtain a heterotrophic nitrification-aerobic denitrification strain,a yeast with heterotrophic nitrification-aerobic denitrification ability was isolated and screened from the sludge of aquaculture pond,named as DW-1.The strain was identified as Diutina rugosa by morphological observation and 26S rDNA sequence analysis.The effects of carbon sources,C/N,initial pH value,cultural temperature and shaking speed on the nitrogen removal performance of strain DW-1 were preliminarily discussed with ammonia nitrogen as the sole nitrogen source.The results showed that the degradation rate of ammonia nitrogen and removal rate of total nitrogen by strain DW-1 were 94.94%and 48.69%respectively,under the conditions of sodium acetate as the sole carbon source,C/N 25,pH 6.0,appropriate culture temperature 32℃and rotate speed 170 r/min;while the accumulation of nitrite nitrogen in the whole process was only 0.067 mg/L.The heterotrophic nitrification-aerobic denitrification performance of D.rugosa DW-1 indicates that it has a promising application prospect in the treatment of nitrogen-containing wastewater.