烟草水提物对果蝇寿命及有关基因转录的影响

探究烟草的水浸泡液对果蝇寿命与衰老情况的影响以及其中的分子机制。取羽化12 h内的新生W^1118系雄果蝇,分别培养于添加不同浓度烟草浸出液的培养基中处理,每日统计果蝇死亡情况;每7日提取对照组与最高浓度处理组果蝇全RNA,通过Real-time PCR检测过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、去泛素化酶(Rpn11)和去乙酰化酶(Sirt6)等与寿命有关基因的表达水平变化。结果显示,烟草影响使雄果蝇寿命显著缩短,且影响随烟草提取液浓度增加影响逐渐增大;烟草影响下果蝇的抗氧化基因出现显著上调,泛素化调节基因与去乙酰化基因等表达水平的变化均出现显著改变。表明烟草对果蝇的寿命情况有不利影响,可能通过引起氧化胁迫导致果蝇早衰。

Effects of Tobacco Extracts on the Lifespan of Drosophila melanogaster and Related Gene Transcription

It aims to explore the effects of tobacco extract on the lifespan and aging of fruit flies and the possible molecular mechanisms.The young W^1118 male fruit flies within 12 h of emergence were cultured in medium supplemented with different concentrations of tobacco extract,and the death of fruit flies was counted daily.The total RNA was extracted from control group and the highest concentration treatment group at every 7 d,and the expressions of life-related genes,such as catalase(CAT),superoxide dismutase(SOD),deubiquitinating enzyme(Rpn11),and deacetylase(Sirt6),were detected by real-time PCR.The result showed that the effect of tobacco significantly shortened the lifespan of male Drosophila melanogaster,which gradually increased with the increase of tobacco extract concentration.The antioxidant genes of the fruit flies were significantly up-regulated under the influence of tobacco,and the changes in the expressions of ubiquitination regulatory gene and deacetylation gene significantly varied.This indicates that tobacco adversely affects the lifespan of fruit flies,which may lead to premature senescence of fruit flies by causing oxidative stress.