发酵法生产制备番茄红素的工艺研究

番茄红素是一种类胡萝卜素,存在于多种果蔬中,具有较好的抗氧化、抗衰老、提高免疫力等功能,被广泛应用于医药、食品、化妆品等领域。然而,传统的提取方法番茄红素产量低、成本高且受制于原料来源,化学合成法存在安全隐患。微生物发酵法因具有工艺简单、生产效率高、生物活性与天然植物提取物一致等优点,逐渐受到关注。基于此,以番茄红素产量为响应值,在单因素实验的基础上利用响应面法优化代谢控制条件,考察正负菌株接种比例、代谢流抑制剂和外源性植物油对番茄红素产量的影响。结果表明,最优条件为三孢布拉氏霉菌负正菌株接种比例6∶1,番茄红素环化酶抑制剂2,6-二甲基吡啶添加量0.09g·L-1,以3.57g·L-1大豆油为外源性植物油辅助发酵,在该最优条件下发酵液中的番茄红素产量达1.51g·L-1,菌体生物量51g·L-1。研究结果显著提高了发酵菌体生物量,促进了番茄红素的生物合成转化率,为番茄红素的工业化生产提供了理论基础。

Study on Fermentation Process of Lycopene Production

Lycopene is a kind of carotene, which exists in many fruits and vegetables. It has good antioxidant, anti-aging and immunity enhancing functions. It is widely used in medicine, food, cosmetics and other fields. However, the traditional extraction method of lycopene has the disadvantages of low yield and high cost, and it is limited by the source of raw materials. Chemical synthesis method has potential safety hazards. Microbial fermentation has attracted increasing attention due to its advantages of simple process, high production efficiency, and the same biological activity as natural plant extracts. Based on this, taking lycopene yield as response value, response surface methodology was used to optimize metabolic control conditions on the basis of single factor experiments, and the effects of positive and negative strain inoculation ratio, metabolic flow inhibitor and exogenous vegetable oil on lycopene yield were investigated. The results showed that the optimal conditions were as follows: the inoculation ratio of the negative and positive strain of Braxella trisporus was 6∶1, the addition of lycopene cyclase inhibitor 2,6-dimethylpyridine was 0.09 g·L-1, and 3.57 g·L-1 soybean oil was used as exogenous vegetable oil. Under the optimal conditions, the lycopene yield in the fermentation broth reached 1.51 g·L-1, and the bacterial biomass was 51 g·L-1. The research significantly increased the biomass of fermented bacteria, promoted the conversion rate of lycopene biosynthesis, and provided a theoretical basis for the industrial production of lycopene.