| 通过诱导剂作用于启动子的条件性基因表达(英文) |
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| 引用本文： | 罗晓艳,唐巍,Vanessa Samuels.通过诱导剂作用于启动子的条件性基因表达(英文)[J].基因组蛋白质组与生物信息学报,2002(1). |
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| 作者姓名： | 罗晓艳 唐巍 Vanessa Samuels |
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| 作者单位： | 北卡罗来纳大学细胞和发育生物学系，北卡罗来纳州立大学生物技术组，北卡罗来纳州立大学生物技术组 查珀尔希尔，北卡罗来纳27599，USA，罗利，北卡罗来纳27695，USA，罗利，北卡罗来纳27695，USA |
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| 摘 要： | 通过遗传工程技术获得的转基因动植物对分析某些生化过程和发育途径极为有用。通过化学诱导剂作用于启动子的条件性基因表达是分子生物学和生物技术应用研究中的强有力的手段。建立于目标基因激活和失活基础之上的几个化学分子诱导基因表达系统已有报道。将来自于原核生物、昆虫和其它动物的调节因子应用于新的物种有利于促进转基因技术的应用和有关基因的时空表达研究。本文综述了有关的基因表达调节系统 ,启动子激活的基因表达系统 ,启动子失活的基因表达系统 ,以及可诱导的基因过度表达和反义抑制系统
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| 关 键 词： | 条件性基因表达 启动子诱导系统 基因过度表达和反义抑制 |
Regulated Gene Expression with Promoters Responding to Inducers |
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| Abstract: | Genetically engineered transgenic animals and plants have proven to be extremely useful for analyzing biochemical and developmental processes.Promoters responding to chemical inducers will be powerful tools for basic research in molecular biology and biotechnological applications.Various chemical inducible systems based on activation and inactivation of the target gene had been described.The transfer of regulatory elements from prokaryotes,insects,and mammals has opened new avenues to construct chemically inducible promoters that differ in their ability to regulate the temporal and spatial expression patterns,and this will dramatically increase the application of transgenic technology.This review provides an overview on regulation of gene expression,promoter activating systems,promoter inactivation systems,inducible gene over expression,and inducible anti suppression. |
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| Keywords: | regulated gene expression inducible promoter systems gene over expression and anti suppression |
| 本文献已被 CNKI 等数据库收录！ |
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