Expression of small RNAs by Bacillus sp. strain PS3 and B. subtilis cells during sporulation |
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Authors: | Pamela S Fink Armando Soto GScott Jenkins Kathleen S Rupert |
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Institution: | Department of Microbiology and Immunology, 002 Mathematics and Microbiology Bldg., Wright State University, 3640 Col. Glenn Hwy., Dayton, OH 45435-0002, USA |
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Abstract: | A small RNA sequence identified in an rRNA-tRNA cluster from the thermophilic Bacillus sp. strain PS3 was examined. An oligonucleotide probe specific for the RNA bound to multiple restriction fragments in Bacillus sp. strain PS3 DNA, thus several copies of this sequence occur in its genome. Similar findings were observed using DNA from B. subtilis, B. stearothermophilus, Escherichia coli, Staphylococcus aureus, Haemophilus influenzae and Thermus thermophilus. This sequence apparently is widespread in the eubacteria. Northern analysis of RNA from sporulating Bacillus sp. strain PS3 and B. subtilis cells revealed RNA species homologous to the probe in both bacteria. Expression of the small RNA in B. subtilis depended on σH. |
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Keywords: | Bacillus Small RNA Sporulation spo0H |
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