High level expression of a heterologous protein in Lactobacillus plantarum and its effect on the persistence of the recombinant strain in silage |
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| Authors: | Jane E Rixon Richard Sharp Anthony G O'Donnell Geoffrey P Hazlewood John K Brehm Nigel P Minton Harry J Gilbert |
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| Institution: | (1) Department of Biological and Nutritional Sciences, The Agriculture Building, University of Newcastle upon Tyne, NE1 7RU Newcastle upon Tyne, UK;(2) Department of Agricultural and Environmental Science, The University of Newcastle upon Tyne, NE1 7RU Newcastle upon Tyne, UK;(3) Department of Cellular Physiology, The Babraham Institute, CB2 4AT Babraham, Cambridge, UK;(4) Centre for Applied Microbiology and Research, SP4 0JG, Porton Dawn, Salisbury, UK |
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| Abstract: | Summary This paper reports the high level expression of the Staphylococcus aureus cat gene in Lactobacillus plantarum using the expression vector pMTL500F. When the recombinant strain of L. plantarum was grown in pure culture, CAT contributed 1.4% of the total soluble cell protein. The recombinant strain of L. plantarum continued to express a high level of CAT when inoculated into silage, the heterologous protein constituting up to 1.75% of the total soluble cell protein. The recombinant L. plantarum strain was still able to survive and proliferate when inoculated into silage, despite its additional metabolic load. |
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