| 尼罗罗非鱼染色体的C带、Ag带和减数分裂联会复合体的研究 |
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| 引用本文： | 王蕊芳,马昆,施立明,贺维顺.尼罗罗非鱼染色体的C带、Ag带和减数分裂联会复合体的研究[J].动物学研究,1990(4). |
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| 作者姓名： | 王蕊芳 马昆 施立明 贺维顺 |
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| 作者单位： | 中国科学院昆明动物研究所
(王蕊芳，马昆，施立明)，中国科学院昆明动物研究所(贺维顺) |
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| 摘 要： | 以Sumner法和界面铺张——硝酸银技术,对尼罗罗非鱼(Tilapia nilotica)染色体C带、Ag染带及减数分裂前期精母细胞联会复合体(SC)进行了显微和亚显微结构观察。 尼罗罗非鱼的2n=44,核型可分为三个组:第一组为4对亚中着丝粒染色体;第二组为17对亚端着丝粒染色体;第三组为具1对端着丝粒的特大染色体。 结构异染色质主要分布于着丝粒附近,其中Nos.6、8、15亚中着丝粒染色体短臂全部深染。带有银染核仁组织者(Ag-NORs)染色体的数目为2—6条,NORs均位于6、8、15亚中着丝粒染色体短臂。 银染色可清楚地显示尼罗罗非鱼的联会复合体(SC)结构和减数分裂行为。SC组型与有丝分裂染色体的组型有较好的一致性。
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| 关 键 词： | 尼罗罗非鱼 染色体组型 核仁组织者 C—带 联会复合体 |
STUDIES ON C-, AG-BANDING CHROMOSOMES AND MEIOTIC SYNAPTONEMAL COMPIEX OF Tilapia nilotica |
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| Wang Ruifang Ma Kun Shi Liming He Weishun.STUDIES ON C-, AG-BANDING CHROMOSOMES AND MEIOTIC SYNAPTONEMAL COMPIEX OF Tilapia nilotica[J].Zoological Research,1990(4). |
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| Authors: | Wang Ruifang Ma Kun Shi Liming He Weishun |
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| Abstract: | C-, Ag-banding chromosomes and synaptonmal complex (SC) of Tilapia nilotica have been reported. The conventional karyotype of Tilapia nilotica consists of 22 pairs of chromosomes and can be classified into three groupsj 4 pairs are submetacentric (Nos. 6,8, 15, 21); 17 pairs subtelocentric (Nos. 2,3, 4, 5, 7, 9,10, 11, 12, 13, 14, 16, 18, 19, 20, 22) and a pair telocentric (No. 1). The C-bands located mainly at the centromeric regions and Nos. 6, 8, 15 chromosomes have been darkly stained on the short arm. Two to six NORs are distributed on the short arm of submetacentric (Nos. 6, 8, 15). Twenty-two SC are formed during the pacytenc stage and no heteromorphic chromosomes have been found. The SC karyotype of Tilapia nilotica also has been constructed. |
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| Keywords: | Tilapia nilotica Karyotype Nucleolus organizer regions Cbanding pattern Synaptonemal complex |
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