Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria |
| |
Authors: | M Ozkan SG Desai Y Zhang DM Stevenson J Beane EA White ML Guerinot LR Lynd |
| |
Affiliation: | (1) Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA, US;(2) Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA, US |
| |
Abstract: | Characteristics of 13 newly isolated thermophilic, anaerobic, and cellulolytic strains were compared with previously described strains of Clostridium thermocellum: ATCC 27405 and JW20 (ATCC 31549). Colony morphology, antibiotic sensitivity, fermentation end-products, and cellulose degradation were documented. All 13 strains were sensitive to erythromycin (5 μg/ml) and chloramphenicol (25 μg/ml), and all strains but one were sensitive to kanamycin (20 μg/ml). Polymerase chain reaction (PCR) amplification using primers based on gene sequences from C. thermocellum ATCC 27405 was successful for all 13 strains in the case of the hydrogenase gene and 11 strains in the case of phosphotransacetylase/acetate kinase genes. Ten strains amplified a product of the expected size with primers developed to be specific for C. thermocellum 16SrRNA primers. Two of the 13 strains did not amplify any product with the PCR primers designed for the phosphotransacetylase/acetate kinase and 16SrRNA primers. A MboI-like GATC- recognizing restriction activity was present in all of the five strains examined. The results of this study have several positive implications with respect to future development of a transformation system for cellulolytic thermophiles. Journal of Industrial Microbiology & Biotechnology (2001) 27, 275–280. Received 12 September 2000/ Accepted in revised form 20 November 2000 |
| |
Keywords: | : cellulolytic anaerobic thermophilic Clostridium thermocellum 16SrRNA |
本文献已被 SpringerLink 等数据库收录! |
|