| 感染柑桔裂皮病类病毒的悬浮细胞的一些特性 |
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| 引用本文： | 刘瑜,赵鸿燕,周咏芝,陈雅丽,丁达明.感染柑桔裂皮病类病毒的悬浮细胞的一些特性[J].Virologica Sinica,1989(3). |
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| 作者姓名： | 刘瑜 赵鸿燕 周咏芝 陈雅丽 丁达明 |
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| 作者单位： | 中国科学院武汉病毒研究所 武汉 |
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| 摘 要： | 爪哇三七(Gynura aurantiaca D.C.)是柑桔裂皮病类病毒(Citrus Exocortis Vi-roid,简称CEV)敏感的指示植物。我们建立了健康和CEV感染的爪哇三七悬浮细胞培养的体外系统。绘制了悬浮细胞培养的生长曲线、pH曲线和温度曲线。CEV可以在悬浮细胞中复制。对继代培养中CEV和寄主核酸的连续测定表明CEV的扩增阻遏了寄主核酸的复制。
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| 关 键 词： | 柑桔裂皮病类病毒 悬浮细胞 负相关 |
Some Properties of Cell Suspension Cultures Containinq Citrus Exocortis Viroid |
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| Lin Yu,Zhao Hong-yan,Zhou Yong-zhi,Chen Ya-li,Ding Da-ming Wuhan Institute of Virology,Academia Sinica,Wuhan.Some Properties of Cell Suspension Cultures Containinq Citrus Exocortis Viroid[J].中国病毒学(英文版),1989(3). |
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| Authors: | Lin Yu Zhao Hong-yan Zhou Yong-zhi Chen Ya-li Ding Da-ming Wuhan Institute of Virology Academia Sinica Wuhan |
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| Abstract: | Gynura aurantiaca D. C. i the sensitive indicator plant of citrus exoc-ortis viroid (CEV). Cell suspension cultures from healthy and CEV-infectedGynura aurantiaca D. C. have been established. Some properties of the cellsuspension cultures have been described by means of growth, pH and temperaturecurves respectively. CEV cln replicate in cell suspension cultures. The cons-istent detection of CEV and host nucleic acids, through successive subcultures,suggest that the amplification of CEV inhibit the replication of bost nucleicacids. |
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| Keywords: | Citrus exocortis viroid Cell suspension Negative relation |
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