Replicating instabilities in yeast: occurrence in different mutational systems |
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| Authors: | A Nasim E R Stephen and J A Erratt |
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| Institution: | (1) Molecular Genetics Section, Division of Biological Sciences, National Research Council of Canada, K1A 0R6 Ottawa, Canada |
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| Abstract: | Summary Following mutagenesis of yeast cells with nitrosoguanidine, primary mosaic colonies exhibiting prototrophic/auxotrophic phenotypes were obtained. Upon replating of these primary mosaics, numerous secondary mosaics were present in the progeny. This study shows that replicating instabilities occur at many different loci within the Schizosaccharomyces pombe genome. In addition, the ade-1 gene of Saccharomyces cerevisiae (causing red pigmentation) was used to show that the phenomenon also occurs in this yeast.NRCC#240/8 |
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