In vitro and in vivo osteogenic activity of licochalcone A |
| |
Authors: | Kim Soon Nam Bae Su Jung Kwak Han Bok Min Yong Ki Jung Seung-Hyun Kim Cheol-Hee Kim Seong Hwan |
| |
Institution: | (1) Laboratory of Chemical Genomics, Pharmacology Research Center, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong-gu, Daejeon, 305-600, Republic of Korea;(2) Department of Anatomy, School of Medicine, Wonkwang University, Chonbuk, Iksan, 570-749, Republic of Korea;(3) Department of Biology and GRAST, Chungnam National University, Daejeon, 305-764, Republic of Korea; |
| |
Abstract: | We investigated the in vitro and in vivo osteogenic activity of licochalcone A. At low concentrations, licochalcone A stimulated
the differentiation of mouse pre-osteoblastic MC3T3-E1 subclone 4 (MC4) cells and enhanced the bone morphogenetic protein
(BMP)-2-induced stimulation of mouse bi-potential mesenchymal precursor C2C12 cells to commit to the osteoblast differentiation
pathway. This osteogenic activity of licochalcone A was accompanied by the activation of extracellular-signal regulated kinase
(ERK). The involvement of ERK was confirmed in a pharmacologic inhibition study. Additionally, noggin (a BMP antagonist) inhibited
the osteogenic activity of licochalcone A in C2C12 cells. Licochalcone A also enhanced the BMP-2-stimulated expression of
various BMP mRNAs. This suggested that the osteogenic action of licochalcone A in C2C12 cells could be dependent on BMP signaling
and/or expression. We then tested the in vivo osteogenic activity of licochalcone A in two independent animal models. Licochalcone
A accelerated the rate of skeletal development in zebrafish and enhanced woven bone formation over the periosteum of mouse
calvarial bones. In summary, licochalcone A induced osteoblast differentiation with ERK activation in both MC4 and C2C12 cells
and it exhibited in vivo osteogenic activity in zebrafish skeletal development and mouse calvarial bone formation. The dual
action of licochalcone A in stimulating bone formation and inhibiting bone resorption, as described in a previous study, might
be beneficial in treating bone-related disorders. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|