Low activity of amine-oxidases and accumulation of conjugated polyamines in disfavour of organogenic programs in Chrysanthemum leaf disc explants |
| |
Authors: | Aribaud Marcel Kevers Claire Martin-Tanguy Josette Gaspar Thomas |
| |
Institution: | (1) Rue de Griffoulet, 17, F-31500 Toulouse, France;(2) Institut de Botanique, Université de Liège, Sart Tilman, B 22, B-4000 Liège, Belgium;(3) Laboratoire de Biologie végétale, Université de Rennes, F-35042 Rennes, France |
| |
Abstract: | Foliar discs (8 mm diameter) from expanding leaves of the middle part of vegetative shoots of Chrysanthemum morifolium Ramat
raised in vitro were induced to form directly on specific media in vitro either roots or vegetative buds, or callus. The budding
programme, on its specific medium, was deviated to callus formation by the addition of 2 mM β-OH-E (β-OH-ethyldrazine, an
inhibitor of diamine oxidase). Conversely vegetative buds instead of callus were formed on the callus medium in the presence
of 2 mM DFMO (difluoromethylornithine, an inhibitor of ornithine decarboxylase). Callus formation was characterized by high
accumulation of free and particularly conjugated polyamines (PA), very low or undetectable activities of diamine- and polyamine
oxidases, and transglutaminase. DFMO-deviation of callus initiation in favour of bud formation lowered the accumulation of
PA and increased the activity of amine-oxidases. The high catabolism of PA in the organogenic (rooting, budding) programs
was questioned as to its role in developmental processes.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | budding callus polyamine anabolism polyamine catabolism rooting transglutaminase |
本文献已被 SpringerLink 等数据库收录! |
|