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Analysis of phospholipid molecular species |
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| Authors: | Vecchini Alba Panagia Vincenzo Binaglia Luciano |
| Affiliation: | (1) Department of Biochemistry, University of Perugia, 06100 Perugia, Italy;(2) Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, Winnipeg, R2H 2A6, Canada |
| Abstract: | A method is described for analysing molecular species of glycerophospholipids. Diglycerides obtained by phospholipase C-catalysed hydrolysis of the phospholipid are separated into the diacyl- alkylacyl- and alkenylacyl- subclasses by HPLC on silicic acid. The molecular species of diacylglycerol are separated by HPLC of underivatised diglycerides on a reverse phase octadecyl-silica column. |
| Keywords: | phospholipid membranes diacylglycerol high performance liquid chromatography |
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